可溶性总神经纤毛蛋白-1检测试剂盒(酶联免疫法)
Total Soluble Neuropilin-1 ELISA
全球首家推出由表位定位特异性抗体定量检测
nNrp-1在神经系统中与其配体 semaphorins (信号素）结合而在轴突中起作用 ， Nrp-1 ， Nrp-2为 Sema受体并且仅与III型Sema结合。
应用领域 ：骨学 ，肿瘤学，肾脏学 ，心血管 。
试剂盒信息
试剂盒信息
货号 |
BI-20409 |
方法学 |
夹心 ELISA, HRP/TMB, 12x8人份测试 |
样本类型 |
血清 、血浆(EDTA, 肝素,枸橼酸钠) 、细胞培养液 |
标准品范围 |
0-12 nmol/l (7个标准品） |
标准品 |
0/0.37/0.75/1.5/3/6/12 nmol/l |
质控品 |
2个 |
样本量 |
10 μl /测试 |
孵育时间 |
30 min/2h/1h/30min |
单位换算 |
1ng/ml = 0.014nmol/l (MW: 69.7 kDa) |
灵敏度 |
LOD: 0.09 nmol/l (0 pmol/l + 3 SD); LLOQ: 0.09nmol/l |
精密度 |
批内差 (n=6) ≤ 11%, 批间差 (n=12) ≤ 10% |
健康人群参考范围 |
Median 血清( n=24): 2.0nmol/l. Median EDTA 血浆(n=24): 1.7nmol/l. Median 肝素 血浆(n=24): 1.7nmol/l. Median枸橼酸钠血浆(n=24): 1.9nmol/l. |
非人属种Nrp-1 |
猴子 ，猪 ，大鼠及小鼠与人Nrp-1同源性超过90% ，本试剂并未对非人属种样本做验证 ，但是大鼠及小鼠的加标回收率以及线性数据表现合格 。
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参考文献
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